TABLE 2

lin-15A overexpression rescues the lin-36(lf); lin-15A(lf), but not the lin-56(lf); lin-36(lf), synMuv phenotype

GenotypeTransgeneLineHeat shock% Muva (n)b
lin-36(n766); lin-15A(n767)None99.6 (251)
lin-36(n766); lin-15A(n767)Phs-lin-15A1100 (39)
+16 (96)
299 (86)
+12 (93)
lin-56(n2728); lin-36(n766)None99.6 (234)
lin-56(n2728); lin-36(n766)Phs-lin-15A1100 (177)
+99.8 (605)
299.6 (227)
+100 (675)
  • lin-36(n766); lin-15A(n766) or lin-56(n2728); lin-15A(n766) hermaphrodites with or without an extrachromosomal array of lin-15A cDNA (Phs-lin-15A) fused to the C. elegans heat-shock promoters and a GFP+ marker were synchronized by bleaching and starvation-induced L1 arrest. Expression from the heat-shock promoters was induced at the early L2 stage (24–25 hr post starvation) by incubation at 33° for 1 hr.

  • a % Muv, percentage of animals that were Muv.

  • b n, number of animals examined.