TABLE 2

New reporter transgenes used in this study

NameTandem constructOperator5′-UTR IVSReporter3′-UTR WPRE
pJFRC-MUH10XUAS
pJFRC110XUASmCD8∷GFP
pJFRC210XUAS+mCD8∷GFP
pJFRC31XUAS+mCD8∷GFP
pJFRC43XUAS+mCD8∷GFP
pJFRC55XUAS+mCD8∷GFP
pJFRC615XUAS+mCD8∷GFP
pJFRC720XUAS+mCD8∷GFP
pJFRC840XUAS+mCD8∷GFP
pJFRC9TH110XUAS+mCD8∷GFP
10XUAS+mCD8∷GFP
pJFRC10THS210XUAS+mCD8∷GFP
10XUAS+mCD8∷GFP
pJFRC11TTS310XUAS+mCD8∷GFP
10XUAS+mCD8∷GFP
pJFRC1210XUAS+myr∷GFPa
pJFRC1310XUAS+GFPa
pJFRC1410XUAS+GFPa+
pJFRC1513XLexAop2mCD8∷GFP
pJFRC1616XLexAop2mCD8∷GFP
pJFRC1726XLexAop2mCD8∷GFP
pJFRC188XLexAop2mCD8∷GFP
  • Janelia Farm Reporter Construct (JFRC) backbones are derived from pBDP (Pfeiffer et al. 2008) and contain the pUC19-derived bacterial origin of replication and ampicillin resistance gene, the PhiC31 attB site, and the mini-white marker for identification of transformants in Drosophila. For more details see Figure 4 and Addgene plasmid 17566. In addition, all vectors also contain a basal promoter derived from hsp70 and an SV40 transcriptional terminator (Brand and Perrimon 1993). Abbreviations: IVS, intervening sequence within the 5′-UTR; and WPRE, a woodchuck hepatitis virus post-transcriptional regulatory element within the 3′-UTR.

  • a Drosophila codon-optimized GFP.