TABLE 2

Analysis of disomy and monosomy in spores derived from the triploid JSC2-1

Chromosomes
SporeIIIIIIIVVVIVIIVIIIIXXXIXIIXIIIXIVXVXVI
5aJaJ YYYbYS YS YJ YS JJS JYSJ YS YS J
5bS YSS JSS JJJSYS YYS JJ YSJY
5cS YSS JSS JJJSYS YYS JJ YSJY
5dJJ YYJ YYS YS YJ YS JJS JYSJ YS YS J
6aJJYSSJaJSJ YJJ YYYS YSS
6bS YS YS JJ YJ YS YS YJ YSS YSS JS JJJ YJ Y
6cJJYSSJJSJ YJJ YYYS YSS
6dS YS YS JJ YJ YS YS YJ YSS YSS JS JJJ YJ Y
8aSS JJ YJ YJJSS JYS YJYaS JS YSS J
8bJ YYSSS YS YJ YYS JJS YS JYJJ YY
8cJ YYSSS YS YJ YYS JJS YS JYJJ YY
8dSS JJ YJ YJJSS JYS YJJcS JS YSS J
9aYS JJSSS YS JJS YYS JS JSS JS YJ Y
9bS JYS YJ YJ YJYS YJS JYYJ YYJS
9cS JYS YJ YJ YJYS YJS JYYJ YYJS
9dYS JJSSS YSbJS YYS JS JSS JS YJ Y
10aJ YJ YJ YJJ YSS YYJS YJS JSS JJJ Y
10bJdYYbJbJJ YSS YYJS YJS JSJbJYb
10cSSSS YSJ YJS JS YJS YYaJ YYS YS
10dSSSYbSJ YJS JS YJS YYYbYS YS
11aS YYYJS YSS JYJS YYYYS YSJ Y
11bJS JS JS YJJ YYS JS YJS JS JS JJJ YS
11cJS JS JS YJJ YYS JS YJS JS JS JJJ YS
11dS YYYJS YSS JYJS YYYYS YSJ Y
12aYS JJJJS YYS YS YSS JSJS YS YS J
12bYS JJJJS YYSabS YSS JSJS YS YS J
12cS JYS YS YS YJSbJJJ YYJ YS ScJJY
12dS JYS YS YS YJS JJJJ YYJ YS ScJJY
13aJS JSS JS YYJ YS JJSJS YSJS YS
13bS YYJ YYJS JSYS YJ YS YJJ YS YJJ Y
13cS YYJ YYJS JSYS YJ YS YJaJ YS YJJ Y
13dJS JSS JeS YYJ YS JJSJS YSJYbeS
14aJYS JSYJSYJJYS JSSS YS
14bJYS JSYJSYJJYS JSSS YS
14cS YS JYJ YS JS YJ YS JS YS YS JYJ YJ YJJ Y
14dS YS JYJ YS JS YJ YS JS YS YS JYJ YJ YJJ Y
15aS YS JS JYJS JS JSJJJJ YJ YS YYJ Y
15bJYYS JS YYYJ YS YS YS YSSJS JS
15cJYYS JS YYYJ YS YS YS YSSJS JS
15dS YS JS JYJS JS JSJJJJ YJ YS YYJ Y
Averagef65.95.95.8665.85.966665.95.95.95.9
  • Using SPA (described in text), we determined whether spores were monosomic or disomic, and the source of each homolog (S, S288c; J, JAY291; Y, YJM789).

  • a Extra chromosome homolog detected by CGH microarrays. These extra chromosomes were not counted in the averages shown at the bottom of this table.

  • b Chromosome loss.

  • c Putative gene conversion event.

  • d Boldface indicates that homologs were detected using PCR and primers that exclusively amplified only one of the strain-specific SNPs.

  • e Translocation between chromosome IV and XV detected by CGH microarray and gel analysis.

  • f Average number of individual homologs per tetrad.