TABLE 2

Loss of mett-10 function leads to multiple, temperature-sensitive phenotypes rescued by maternal gene product

GenotypemaTemperatureb% Pvl% Ste% Meln
mett-10(oz36)No20140116
mett-10(oz36)No2543100NA103
mett-10(oz36)Yes25039758
mett-10(g38)No2027059
mett-10(g38)No2521891163
mett-10(g38)Yes250109021
mett-10(tm2697)No2010190133
mett-10(tm2697)No2588100NA142
mett-10(tm2697)Yes25029860
mett-10(oj32)No20018071
mett-10(oj32)No2511100NA75
mett-10(oj32)Yes250010053
mett-10(ok2204)No20128052
mett-10(ok2204)No259899NA82
ozIs7[mett-10∷GFP]; mett-10(ok2204)No2550038
mett-10(ok2204)Yes250010045
  • Pvl, protruding vulva; Ste, Sterile (no embryos present); Mel, maternal-effect lethal (lays dead embryos); NA, not applicable, by definition, since sterile mutants lay no embryos and thus cannot be Maternal-effect lethal.

  • a m, endogenous maternal wild-type gene product.

  • b All animals were scored 1 day past the L4 stage by DIC.