TABLE 1

ot119 and ot201 specifically affect cog-1 function in the ASER neuron but not in other cell types

Canonical cog-1 mutant phenotypes
Genotype% animals with ASER defect (class I Lsy phenotype = ectopic lim-6∷gfp expression)% animals with VulB2 defect (loss of ceh-2∷gfp expression)% animals with Egl defecta% animals with Pvl defectb
Wild type0 (n > 100)0 (n = 58)0 (n = 30)0 (n = 30)
ot11963 (n = 36)c0 (n = 24)0 (n = 30)0 (n = 30)
ot20189 (n = 54)c0 (n = 27)0 (n = 30)0 (n = 30)
sy607d100 (n = 35)c90 (n = 20)e100 (n = 30)f87 (n = 30)f
ot119/sy60790 (n = 20)11 (n = 19)0 (n = 30)0 (n = 30)
ot201/sy60735 (n = 24)3 (n = 32)0 (n = 30)0 (n = 30)
  • Markers used are otIs114 (lim-6∷gfp) (Chang et al. 2003) and syIs54 (ceh-2∷gfp) (Inoue et al. 2005).

  • a Defined as animals with reduced brood size, including the bag-of-worms phenotype.

  • b Pvl, protruding vulva phenotype.

  • c This phenotype has already been described in Sarin et al. (2007); animals have been newly scored with similar results.

  • d sy607 is a strong loss-of-function or null allele of cog-1 that affects its coding region (Palmer et al. 2002).

  • e Similar to results reported by Inoue et al. (2005).

  • f Similar to results reported by Palmer et al. (2002).