TABLE 2

Genotoxic stress phenotypes for precise heterozygous gene deletions

Heterozygous deletionStrain backgroundUVMMSBenomylPhleomycinHU
ATG11Wild typeAll
ATG11rad9Δ/rad9ΔResistantResistant
ARO4Wild typeAll
ARO4rad9Δ/rad9ΔAllMildResistantMild
ACK1rad9Δ/rad9ΔMild
CHS3Wild typeAllAllAllAllAll
DUN1Wild typeAll-mildMildAllAll
GDA1Wild typeAll
GDA1rad9Δ/rad9ΔAllMildResistantAll
HIR3rad9Δ/rad9ΔResistantResistantMild
HOP1Wild typeAll
HOP1rad9Δ/rad9ΔAllMildResistant
MRC1Wild typeAll
MRC1rad9Δ/rad9ΔResistant
MCM21Wild typeAllMildResistantMild
NDD1Wild typeAllMildMild
NGR1Wild typeMildMildResistant
NGR1rad9Δ/rad9ΔAllMildResistant
PPH21Wild typeMild
RAD1Wild typeAll
RAD55Wild typeAllAllAllAll
RRN3Wild typeMild
SAM1Wild typeResistantResistantResistant
SAM1rad9Δ/rad9ΔResistant
SIR4Wild typeAll
SPE1Wild typeResistantResistantResistant
TMA17Wild typeResistantResistant
TRX1Wild typeAll
TRX1rad9Δ/rad9ΔResistant
YAL066WWild typeResistantResistant
YAL066Wrad9Δ/rad9ΔResistant
YBL104CWild typeAllAll
YDR066CWild typeAll
YCR066Crad9Δ/rad9ΔResistantResistant
  • Sixty-five precise heterozygous deletions in rad9Δ/rad9Δ and wild-type backgrounds, as described in Table 1, were tested for response to genotoxic stress. Forty-one of the 65 tested heterozygous gene deletions were not sensitive to any of the agents tested in either background. Strains that showed an altered response to any agent are shown. Exposures to UV doses of 20, 30, and 40 J/m2/sec; methyl methanesulfonate doses of 0.0075, 0.015, and 0.03%; benomyl doses of 10, 20, and 30 μg/ml; phleomycin doses of 0.1, 0.5, and 1 μg/ml; and HU doses of 25, 50, and 75 mm were tested. All, sensitive to all doses; mild, sensitive only at high doses; All-mild, slight decreases in growth seen at every dose.