TABLE 2

Plasmids used in this study

PlasmidRelevant featuresSource or reference
pBAD24Ampr, araBAD promoter-based expression vector, pUC-type oriGuzman et al. (1995)
pBAD30Ampr, araBAD promoter-based expression vector, p15a oriGuzman et al. (1995)
pBAD322AmpR, araBAD promoter-based expression vector, complete pBR322 originCronan (2006)
pCR2.1Ampr, Kanr, cloning vector, pUC oriInvitrogen
pKD4oriRγ, Kanr cassette flanked by FRT sitesDatsenko and Wanner (2000)
pKD46AmpR, RepA1019(Ts), λ, γ, β, and exo expressed from an araBAD promotorDatsenko and Wanner (2000)
pQE70-SfpAmpr, pQE70-derived plasmid encoding Sfp with a C-terminal His6 tagMofid et al. (1999)
pYon113Ampr, acpT under the control of an araBAD promoterFlugel et al. (2000)
pNRD28acpS amplified from JM105 using primers AcpSBAD For and Rev and TOPO cloned into pCR2.1This study
pNRD83EcoRI–HindIII acpS fragment of pNRD28 inserted into the same sites of pBAD322This study
pNRD192EcoRI–XbaI acpS fragment of pQE70-Sfp inserted into the same sites of pBAD30This study
pNRD203EcoRI–HindIII acpS acpS fragment of pNRD83 inserted into the same sites of pBAD24This study
pNRD204EcoRI–XbaI sfp acpS fragment of pNRD192 inserted into the same sites of pBAD18This study
pNRD205Encodes AcpT D91E. Site-directed mutation in acpT in pYon113 was generated using primers AcpTD91E For and Rev.This study
pNRD206Encodes AcpT E137S. Site-directed mutation in acpT in pYon113 was generated using primers AcpTE137S For and Rev.This study
pNRD207Encodes AcpT K141A. Site-directed mutation of acpT in pYon113 using primers AcpTK141A For and Rev.This study
pNRD216yejM amplified from JM105 using primers YejM For and Rev primers and TOPO cloned into pCR2.1This study
pNRD217NcoI–XbaI yejM containing fragment from pNRD216 inserted into the same sites of pBAD322This study