Data on reciprocal crosses among centrarchid species

Species pairHybrid viability (% of control cross)Relative viability differenceWorse damRate asymmetry δiSpecies with δi > 0Worse dam: faster mtDNA?Smaller species
NodeABA × BB × AABSpeciesFaster mtDNA?Worse dam?
AA. rupestrisL. gulosus25.5−0.636A0.002−0.002AYesBNoNo
AA. rupestrisL. macrochirus2650.808B−0.2940.294BYesBYesYes
BA. rupestrisP. nigromaculatus4186.5−0.526A0.029−0.029AYesAYesYes
IL. cyanellusL. gulosus99107−0.075A0.012−0.012AYesAYesYes
HL. cyanellusL. macrochirus105900.143B0.045−0.045ANoEqualNANA
GL. cyanellusL. microlophus1181070.093B−0.1040.104BYesBYesYes
DL. cyanellusM. salmoides1180−0.863A0.062−0.062AYesAYesYes
HL. gulosusL. macrochirus77350.545B0.045−0.045ANoAYesNo
GL. gulosusL. microlophus107820.234B0.224−0.224ANoBNoYes
DL. gulosusM. salmoides75103−0.272A0.062−0.062AYesAYesYes
AL. gulosusP. nigromaculatus1.72.3−0.261A−0.1550.155BNoANoYes
GL. macrochirusL. microlophus77102−0.245A−0.1440.144BNoBYesNo
DL. macrochirusM. salmoides770−0.900A0.324−0.324AYesAYesYes
AL. macrochirusP. nigromaculatus1344−0.705A0.290−0.290AYesAYesYes
DL. microlophusM. salmoides043−1.0A0.399−0.399AYesAYesYes
EM. dolomieuM. salmoides82102−0.196A0.058−0.058AYesAYesYes
FM. floridanusM. salmoides109910.165B−0.1210.121BYesANoNo
CP. annularisP. nigromaculatus98770.214B−0.0030.003BYesBYesYes
  • Node identities correspond to Figure 3. For each species pair we arbitrarily assign a species A and B, which we use to specify the viabilities of the respective reciprocal crosses (female parent listed first). Viabilities are calculated by dividing the percentage of hybrid embryos that survive to larval stage by the percentage of intraspecific control cross embryos that survive. Relative viability difference is calculated as the difference in viabilities, standardized by the higher value. The “worse dam” is the species providing the eggs for the lower viability reciprocal cross. Relative mitochondrial rates, δi, for both species are calculated from branch length data in supplemental Table 1 of the supplemental Appendix ( The species with the higher value exhibits relatively faster mtDNA evolution. Under the heading “Smaller species” we identify the species with the smaller maximum body size and state whether this coincides with having faster-evolving mtDNA or being the worse dam.