TABLE 7

sex-1 regulates her-1 transcript levels

Transcript measured by qRT–PCRa
Genotypeher-1bfasn-1c
xol-1(y9) XX1.2 ± 0.21.0 ± 0.1
sex-1(y424) XX6.5 ± 0.41.1 ± 0.1
xol-1(y9) sex-1(y424) XX4.6 ± 0.71.0 ± 0.1
  • a The levels of her-1 transcripts were measured in embryos of different genotypes (listed by genotype) by qRT–PCR and are expressed as the fold change compared to the transcript levels measured in wild-type XX embryos. For example, a value of 2.0 means that twice as many gene-specific transcripts were measured in mutant embryos than in wild-type XX embryos. All transcripts levels were normalized to the levels of the control gene, nhr-64, whose expression is not affected by dosage compensation. Fatty acid synthase fasn-1, another gene not affected by dosage compensation, was used as a control to gauge the variability and reliability of measurements made using qRT–PCR. See Van Gilst et al. (2005) for protocol. Experimental error is expressed as the standard error of the mean. Similar results were obtained for all genotypes in separate qRT–PCR experiments in which transcript levels were normalized to the levels of fasn-1.

  • b qRT–PCR primers amplify both the short and long transcripts of her-1, which are coordinately regulated in a sex-specific manner despite being produced by two different her-1 promoters. Measurements thus reflect changes in total her-1 transcripts.

  • c A critical control was to compare the nhr-64-normalized fasn-1 transcript levels in three independent preparations of wild-type embryos. That comparison showed the fasn-1 transcript levels to be statistically equivalent among the independent RNA preparations (fasn-1, 1.1 ± 0.2).