TABLE 3

Multiple XSEs must be reduced by half to perturb dosage compensation in XX animals

GenotypeaXX phenotypeHermaphrodite viability (%)bnc
fox-1 +/+ sex-1dWild type100NA
yDf20 [Δ of ceh-39, fox-1]/+dWild type99NA
yDf20 [Δ of ceh-39, fox-1] +/+ sex-1dDpy, Egl, Tra29NA
yDf17 [Δ of region 1, ceh-39, fox-1] +/+ sex-1dDead0NA
ceh-39 + +/+ fox-1 sex-1eWild type or mild Dpy, Egl107f1401
ceh-39 + +/+ sex-2 sex-1gWild type98f1867
ceh-39 fox-1 + +/+ + sex-2 sex-1hDpy, Egl60f1081
  • a Alleles used were sex-1(y263), fox-1(y303), sex-2(y324), and ceh-39(y414).

  • b Hermaphrodite viability was calculated by the formula: [no. of adult hermaphrodites]/[expected no. of hermaphrodites, (0.5)n] × 100.

  • c n is the total number of embryos from at least six independent sets of progeny counts.

  • d Data are from Carmi and Meyer (1999).

  • e Crosses between fox-1 sex-1 XO males and unc-32; ceh-39 hermaphrodites yielded non-Unc cross progeny that ranged from wild type to mild Dpy Egl. The phenotype of ceh-39 + +/+ fox-1 sex-1 animals (Dpy and Egl) is more severe than that of fox-1+/+ sex-1 animals (wild type), showing that changing the dose of ceh-39 by half reduces the overall X signal.

  • f Hermaphrodite viability was calculated as the [no. of adult hermaphrodites (either non-Unc or non-Dpy-3)]/[(0.5)(n − no. of Unc or Dpy-3 adults)] × 100. Unc or Dpy-3 adults represent self-progeny.

  • g Crosses between sex-2 sex-1 XO males and unc-32; ceh-39 hermaphrodites yielded non-Dpy non-Unc cross progeny.

  • h Crosses between sex-2 sex-1 XO males and ceh-39 dpy-3(e27) fox-1 hermaphrodites yielded non-Dpy-3 cross progeny that ranged from Dpy to wild type. The ceh-39 allele gk296 showed results similar to y414.