TABLE 2

fox-1 is a stronger XSE than ceh-39

yDp14/yDp14; him-8 XO + RNAi of geneaMale viability (%)bnc
No RNAi0929
dsRNA from vector with no gened01055
fox-1(RNAi)d691174
ceh-39(RNAi)d41030
fox-1(RNAi)e721083
ceh-39(RNAi)e41060
  • a The relative strength of the XSEs fox-1 and ceh-39 was examined by treating hermaphrodites of genotype yDp14/yDp14 (X;I); him-8(e1489) IV; unc-2(e55) X with dsRNA corresponding to the gene listed, and the viability of progeny males was assessed. RNAi-mediated knockdown of an XSE should decrease the male lethality caused by the increase in XSE dose from yDp14. yDp14 is an X duplication attached to LG I that can exist in one copy (yDp14/+) or two copies (yDp14/yDp14) (Akerib and Meyer 1994). him-8 XX animals produce 37% XO males, 57% XX hermaphrodites, and 6% Dpy XXX hermaphrodites (Hodgkin et al. 1979).

  • b Male viability was calculated by the formula: (no. of adult males)/(expected no. of males) × 100. The number of expected males was (0.37)n.

  • c n is the total number of embryos from at least six independent sets of progeny counts.

  • d Hermaphrodites were injected with dsRNA corresponding to the indicated gene, and the viability of progeny males was assessed.

  • e Hermaphrodites were fed bacteria producing dsRNA corresponding to the indicated gene, and the viability of progeny males was assessed.