TABLE 7

XSE mutations increase xol-1 transcript levels

Transcript measuredby qRT–PCRa
Genotypexol-1nhr-64
sex-1(y263)3.0 ± 0.51.1 ± 0.1
sex-2(y324)1.8 ± 0.21.2 ± 0.1
ceh-39(y414)2.0 ± 0.21.1 ± 0.1
ceh-39(gk296)2.0 ± 0.31.1 ± 0.1
fox-1(y303)1.0 ± 0.11.3 ± 0.1
  • a The levels of xol-1 and XSE transcripts in mutant embryos (listed by genotype) were measured by qRT–PCR and are expressed as the fold change compared to the transcript levels measured in wild-type embryos (see materials and methods). For example, a value of 2.0 means that twice as many gene-specific transcripts were measured in mutant embryos than in wild-type embryos. All transcript levels were normalized to the levels of the control gene, fasn-1 (ORF F32H2.5), whose expression is constant throughout embryogenesis and is not affected by dosage compensation. See Van Gilst et al. (2005) for details and protocol. nhr-64, another gene not affected by dosage compensation, was used as a control to gauge the variability and reliability of measurements made using qRT–PCR. Experimental error is expressed as the standard error of the mean. A critical control was to compare the fasn-1-normalized xol-1 or nhr-64 transcript levels in three independent preparations of wild-type embryos. That comparison showed the xol-1 and nhr-64 transcript levels to be statistically equivalent among the independent RNA preparations (xol-1, 1.2 ± 0.2; nhr-64, 1.3 ± 0.1).