TABLE 4

Increased dose of ceh-39 enhances XO-specific lethality caused by increased XSE dose

XO genotypesNo. of malesMale viability (%)anb
Wild typec8121001632
yIs58[ceh-39(+)]/yIs58d555981131
yDp14/+e22061724
yDp14/+; yIs58[ceh-39(+)]/+f141201440
yDp14/+; yIs58[ceh-39(+)]/yIs58g2231522
  • a Males were generated through crosses and their viability was calculated by the following formula: [no. of adult males]/[the expected no. of males, (0.5)n] × 100. In all crosses, the number of hermaphrodites was 0.5(n), implying that the matings produced only cross progeny and that hermaphrodite viability was 100%.

  • b n is the total number of embryos from six independent sets of progeny counts.

  • c Wild-type males were produced by mating wild-type males and hermaphrodites.

  • d Males were produced by mating yIs58[ceh-39(+)]/yIs58 males and hermaphrodites. yIs58[ceh-39(+)] is an integrated transgenic array carrying multiple copies of a 5.5-kbp genomic fragment spanning the entire ceh-39 locus. Two copies of yIs58 elevate the ceh-39 transcript level fourfold above the wild-type level (Gladden et al. 2007, accompanying article in this issue).

  • e Males were produced by mating wild-type males with yDp14/yDp14; unc-2(e55) hermaphrodites.

  • f Males were generated by mating yIs58[ceh-39(+)]/yIs58 males with yDp14/yDp14; unc-2(e55) hermaphrodites.

  • g Males were generated by mating yIs58[ceh-39(+)]/yIs58 males with yDp14/yDp14; yIs58/yIs58; unc-2(e55) hermaphrodites.