Putatively functional sequences and pseudogenes obtained by cloning the PCR product from three different protocols: standard PCR, high-stringency PCR (with DMSO), and (RT–PCR)

Putatively functionalPseudogenes:
Group 1Group 2Groups 3–8
Standard PCR3923
PCR with DMSO1810
  • The eight groups represent well-supported clades in a maximum-likelihood tree, of which two are putatively functional (groups 1 and 2) and the rest are pseudogenes (Figure 2).