TABLE 2

Summary of phenotypes analyzed, number of animals, and mean age (in days) at which the animals were analyzed

PhenotypeDescriptionNo. of animalsMean age (days)
Weight, 6 wkBody weight at the beginning of testing.251642
ImmunologyCD4, CD3, CD8, and B220 antibody staining.187242
OFTOpen field arena: distance in the perimeter, the center, and total distance in 5 min.250445
EPMElevated plus maze: distance traveled, time spent, and entries into closed and open arms.245246
FNFood hyponeophagia: time taken to sample a novel foodstuff (overnight food deprivation).247447
BurrowingNo. of pellets removed from burrow in 1.5 hr.245548
ActivityActivity measured in a home cage in 30 min.244548
StartleStartle to a loud noise.194852
Context freezingFreezing to the context in which a tone is associated with a foot shock.207055
Cue freezingFreezing to a tone after association with a foot shock.211056
PlethysmographyAnimals sensitized by injection with ovalbumin inhale metacholine and changes in lung function are measured by plethysmography (a model of asthma). Respiratory rate, tidal volume, minute volume, expiratory time, inspiratory time, and enhanced pause are recorded with and without exposure to metacholine.230463
IPGTTGlucose and insulin values taken at 0, 15, 30, and 75 min after i.p. glucose injection (a model of type 2 diabetes mellitus).233468
Weight, 10 wkBody weight at the end of testing.231970
FBCFull blood count (hematocrit, Hb concentration, mean cellular volume, mean cellular Hb concentration, white cell count, platelet count).189271
Tissue harvestAdrenal weight.230971
Wound healingReduction in size of a 2-mm ear punch hole.227371
BiochemistryAlbumin, alkaline phosphatase, alanine transaminase, aspartate transaminase, calcium, chloride, creatinine, high-density lipoprotein, low-density lipoprotein, phosphorous, sodium, total cholesterol, total protein, triglycerides.189071