TABLE 3

Homeologous loci examined in T. miscellus with genomic and cDNA CAPS analysis

Diagnostic restriction enzymeGenomic fragment sizesa (bp)cDNA fragment sizesa (bp)
FragmentT. dubiusT. pratensisT. dubiusT. pratensis
TDF7BstNI44741311477
3737
TDF17.4Sau3AI108132108132
81818181
41414141
24182418
1818
TDF36.3DdeI8816488164
7272
TDF44HpyCH4IV393219339191
(MaeII)91170148
72
21
TDF46NlaIII186277186277
9494
TDF62Sau3AI266371185291
1069710697
9797
TDF72.3AciI289220209140
6969
TDF74NlaIII3454035480
88514545
51826
TDF85AluI305270303264
9214539
39
TDF90BsmAI222222222222
110125110125
1313
  • a Following digestion with diagnostic restriction enzyme.