TABLE 1

List of tested antibodies, their localization, and dilutions used

AntibodySource/catalog no.HostPachytene immunofluorescence (IF) stainingWBDilution for IFDilution for WB
H3K4me2Upstate/07-030RabbitBetween chromomeres+1:501:2,000
H3K9me1Upstate/07-450RabbitPericentromeres, chromomeres, and between chromomeres+1:751:10,000
H3K9me2Upstate/05-768RabbitBetween chromomeres and in/around kinetochores (weak)+1:501:5,000
H3K9me2Upstate/07-212RabbitBetween chromomeres and in/around kinetochores (strong)+1:501:2,000
H3K9me2Upstate/07-441RabbitBetween chromomeres and in/around kinetochores (medium)ND1:50ND
H3K9me3Abcam/ab8898RabbitBetween chromomeres and in/around kinetochores+1:501:2,000
H3K27me1Upstate/07-448RabbitPericentromeres, chromomeres, and continuously between chromomeres+1:1001:12,000
H3K27me2Upstate/07-452RabbitPericentromeres and chromomeres+1:501:2,000
H3K27me2Abcam/ab14222RatPericentromeres and chromomeresND1:50ND
H3K27me3Upstate/07-449RabbitSeveral discrete domains+1:501:1,000
H3K27me3Abcam/ab6002MouseSeveral discrete domains (only stained in W23)ND1:25ND
Acetyl H4Upstate/06-598RabbitND+1:50 (mouse)1:2,500
H4K20me1Upstate/07-440RabbitNo visible signals+1:25–1:2001:2,000
H4K20me2Upstate/07-367RabbitNo visible signals1:25–1:2001:2,000
H4K20me3Upstate/07-463RabbitNo visible signals1:25–1:2001:2,000
H4K20me3Abcam/ab9053RabbitNo visible signalsND1:25–1:200ND
  • ND, no data available; WB, Western blot.