TABLE 2

Kinds of mutations produced in vitro depending on accessory proteins

Nonegp32 ± gp44/62gp45/44/62gp32/45/44/62
ChangeOldNewTotalMFOldNewTotalMFNewMFOldNewTotalMF
Mutations15274226226299525347171214385
 Small14674220158225299524256338245170209379251
 Other6 0  61  0  1  91  5  6
 G → A5415 695027 7910652 825956 5511173
 C → T12 3 15118 13 2110 191421  6 2718
 A → G0 0  0≤0.75  1  63  10.70  0  0≤0.7
 T → C3734 715113813727513514710668 87155103
Transitions10352155112178230408200249180145148293194
 G → T2 5  751  1  21  430  4  43
 G → C4 0  431  3  42  750  2  21
 C → A0 1  10.70  0  0≤0.5  322  2  43
 C → G0 0  0≤0.70  1  10.5  0≤0.70  0  0≤0.7
 A → T2 1  320  0  0≤0.5  211  2  32
 A → C1 0  10.70  0  0≤0.5  0≤0.70  1  10.7
 T → A8 3 1188 10 189  753 11 149
 T → G0 1  10.712  7 199  751  5  64
Transversions1711 282022 22 4422 30227 27 3422
 −G5 2  751  5  63 14100  7  75
 −C4 3  754  8 126 1183 10 139
 −A0 0  0≤0.70  1  10.5  0≤0.70  3  32
 −T6 2  8611 14 2512 201411  7 1812
 −115 7 221616 28 4422 453314 27 4127
 +G0 0  0≤0.71  1  21  10.70  0  0≤0.7
 +C1 1  210  3  31  0≤0.70  0  0≤0.7
 +A4 0  431  4  52  0≤0.70  0  0≤0.7
 +T6 3  967 11 189 1394  7 117
 +111 4 15119 19 2814 14104  7 117
  • Most Old entries are from Bebenek et al. (2002). The New entries are from more recent reaction experiments, Total is the sum of the Old and New values, and MF is the mutation frequency × 104 for the Total column (the product of the pooled MF values from Table 1 times the fraction of mutations of each type). Note, however, that the Old gp32 entries are from reactions performed in the presence of gp44/62 while the New gp32 entries are from reactions with gp32 alone. The Other mutations include complex mutations and indels (mostly deletions) of ≥2 nucleotides and were not included in the spectral analyses. “≤” values are as if 1 mutation had been observed. Numbers in italics are subtotals.