TABLE 1

Yeast strains used in this study

Yeast strainsRelevant genotypeCommon name used
in this work
Reference
YFP17Δhml::ADE1 Δmata::hisG Δhmr::ADE1 ade3::GAL-HO leu2::HOcsa ura3-52Paques et al. (1998)
AGY117YFP17, LEU2 URA3::aib::HOcs (-) orientationWT, one cut site at URA3Yu and Gabriel (1999)
AGY116YFP17, LEU2 URA3::ai::HOcs (+) orientationThis work
AGY245YFP17, URA3::aiWT, one cut site at LEU2This work
AGY113YFP17, URA3::ai::HOcs (-) orientationWT, two cut sitescThis work
AGY127YFP17, LEU2 URA3::ai::HOcs (-) orientation Δrad52::hisGrad52, one cut site at URA3Yu and Gabriel (1999)
AGY126YFP17, LEU2 URA3::ai::HOcs (+) orientation Δrad52::hisGThis work
AGY262YFP17, URA3::ai Δrad52::hisGrad52, one cut site at LEU2This work
AGY121YFP17, URA3::ai::HOcs (-) orientation Δrad52::hisGrad52, two cut sitesThis work
AGY287YFP17, LEU2 URA3::ai::HOcs (-) orientation Δyku80::KanMX4 at URA3yku80, one cut siteYu and Gabriel (2003)
AGY601YFP17, URA3::ai Δyku80::KanMX4yku80, one cut site at LEU2This work
AGY285YFP17, URA3::ai::HOcs (-) orientation Δyku80::KanMX4yku80, two cut sitesThis work
AGY481YFP17, LEU2 URA3::ai::HOcs (-) orientation Δyku80::KanMX4 Δrad52::hisGrad52 yku80, one cut site at URA3Yu and Gabriel (2003)
AGY622YFP17, URA3::ai Δyku80::KanMX4 Δrad52::hisGrad52 yku80, one cut site at LEU2This work
AGY289YFP17, URA3::ai::HOcs (-) orientation Δyku80::KanMX4 Δrad52::hisGrad52 yku80, two cut sitescThis work
  • WT, wild type.

  • a HOcs, HO cut site.

  • b ai, actin intron.

  • c Two cut sites, HO cut sites present at both URA3 and LEU2.