TABLE 4

rpoS::Tn10 does not reduce transductional recombination

Transductant frequency
(×10-4)b
Fold
decrease
Mean fold decrease
(±SEM)
SelectionaExperimentrpoS+rpoS::Tn10
Thr+15.83.41.70.94 ± 0.4
21.22.00.60
31.22.30.52
Trp+10.862.70.321.2 ± 0.8
20.280.102.8
36.1130.47
  • a For threonine or tryptophan prototrophy (materials and methods).

  • b Recipient and donor strains are: rpoS+ thr, SMR6993; rpoS::Tn10 thr, SMR6994; rpoS+ trp, SMR7037; rpoS::Tn10 trp, SMR7038; P1 phage donor, SMR4562. Transductant frequencies are means from two to four transductions of one rpoS+ or rpoS recipient culture at varying MOIs (all <0.01 phage per cell).