TABLE 6

Muk silences MuDR elements independent of their position or number

a1-mum2 minimal Mutator line testeraMuk/–; a1-mum2b
PlantMu1 methylation statusTdF2 spotted kernels (%)PlantMu1 TIR methylation statuscTdF2 spotted kernels (%)
1Hypo1061001Hypo263100
2Hypo2241002Hypo55100
3Hypo571003Hypo141100
4Hypo1821004Hypo167100
5Hypo1751005Hypo186100
6Hypo1971006Hypo145100
7Hypo2201007Hypo179100
8Hypo2811008Hypo30100
9Hypo1741009Hyper2020
10Hypo20310010Hyper2130
11Hypo18210011Hyper3700
12Hypo27210012Hyper1100
13Hypo25010013Hyper2650
14Hypo13910014Hyper2230
15Hypo25510015Hyper2640
16Hypo25910016Hyper940
17Hypo17210017Hyper1130
18Hypo20810018Hyper2240
19Hypo6510019Hyper1990
20Hyper175120Hyper2410
21Hyper70021Hyper1090
22Hyper213022Hyper2810
  • a Generated by crossing the a1-mum2 minimal Mutator line tester by a multiple MuDR-tagging line individual and then crossing the F1 progeny as female to the a1-mum2 minimal Mutator line tester.

  • b Generated by crossing Muk/–; a1-mum2 by the same multiple-MuDR-tagging line individual and then crossing the F1 progeny as female to the a1-mum2 minimal Mutator line tester.

  • c Mu1 TIR methylation status as judged by Southern blot using HinfI digestion and Mu1 probe. Hyper, hypermethylated Mu1 TIRs; Hypo, hypomethylated Mu1 TIRs.

  • d Total number of informative a1-mum2/a1-mum2 kernels on the test-crossed ear.