TABLE 2

Weakly spotted kernels have hypermethylated TIRs even in the presence of MuDR(p1)

NonspottedHeavily spottedWeakly spotted
GenerationTap1bHypercTap1bHypocTap1bHyperc
1d17117636363616155
2Ae12012303030000
2Bf20020151515131213
  • a Total number examined by Southern blot.

  • b resence of full-length MuDR(p1) determined by PCR and Southern blot.

  • c Methylation status as judged by HinfI-digested Southern blots probed with Mu1. Hyper, hypermethylated TIRs; Hypo, hypomethylated TIRs.

  • d Plants used for this analysis were a subset from Table 1, cross 1. The plants were generated from the cross Muk/–; a1-mum2 × MuDR(p1)/–; a1-mum2.

  • e Progeny of an a1-mum2 plant [without both Muk and MuDR(p1)] crossed as female to MuDR(p1)/–; a1-mum2. Plants used for this analysis were a subset from Table 3, cross 3.

  • f Progeny of a Muk/–; a1-mum2 plant [without MuDR(p1)] crossed as female to MuDR(p1)/–; a1-mum2. Plants used for this analysis were a subset from Table 3, cross 14.