TABLE 2

Fitness of diploids constructed from the progenitor haploid and FLC-resistant haploids from experiment 1

Diploids/
progenitora
MIC
FLC
No. of doublings ±SD (n = 3 replicates)
Tag XbTag YbTag 5 (all P5)
Parent diploidsc
P1/P5166.2 ± 0.16.2 ± 0.2
D2/P525614.7 ± 0.05.5 ± 0.4
D3/P525614.1 ± 0.16.6 ± 0.8
D4/P525614.4 ± 0.07.2 ± 1.0
Reconstructed diploids
P1 × P1/P5167.2 ± 0.16.9 ± 0.2
D2 × D2/P525614.5 ± 0.24.3 ± 2.8
D3 × D3/P525614.4 ± 0.27.3 ± 1.5
D4 × D4/P525614.0 ± 0.17.1 ± 1.9
Hybrid diploids (D × D)
D2 × D3/P525614.5 ± 0.114.4 ± 0.18.8e
D2 × D4/P525614.4 ± 0.114.0 ± 0.26.4 ± 1.5
D3 × D2/P525614.4 ± 0.214.5 ± 0.26.7 ± 1.8
D3 × D4/P525614.2 ± 0.114.2 ± 0.17.1 ± 1.0
D4 × D2/P525614.7 ± 0.114.5 ± 0.26.5 ± 1.0
D4 × D3/P525614.4 ± 0.114.4 ± 0.07.2 ± 1.1
Hybrid diploids (P × D)d
P1 × D2/P5648.4 ± 0.18.5 ± 0.17.1 ± 0.1
P1 × D3/P51287.4 ± 0.27.6 ± 0.27.3 ± 0.2
P1 × D4/P5649.2 ± 0.49.2 ± 0.37.5 ± 0.3
D2 × P1/P5648.4 ± 0.38.3 ± 0.27.0 ± 0.6
D3 × P1/P512811.8 ± 0.211.3 ± 0.26.8 ± 0.5
D4 × P1/P5648.8 ± 0.28.9 ± 0.07.1 ± 0.2
  • The mean number of doublings for all diploid strains in 0.5× YPD with no FLC was 13.5 ± 0.2 (data not shown).

  • a Diploids are listed with MATα strain first and the MATa strain second. Progenitor diploid P5 after the slash (/) was the reference strain in each fitness assay.

  • b Fitness of each diploid carrying two tags (e.g., D2 × D3) was measured with both tags (e.g., tag 2 and tag 3).

  • c Parent diploids were constructed from the haploid strains by transient transformation with a plasmid carrying the HO gene.

  • d Because these assays were done at a concentration of FLC that was higher than the measured MIC, the overall density at the end of these competition assays remained low and these measures of fitness were therefore more variable than those done under conditions in which the mixed cultures reached high density at the end of the assay period.

  • e No standard deviation available; only one measurement was made.