TABLE 8

Genetic interactions between glp-1(ar202) and suppressors and enhancers of lin-12 and glp-1

Germline pattern phenotype
Genotype% wild type% Pro% other(n)
glp-1(ar202)a,b0964(44)
glp-1(ar202); sel-12(ar131)c5950(43)
glp-1(ar202); sel-12(ar171)d9910(32)
glp-1(ar202); hop-1(RNAi)a,e19791(89)
glp-1(ar202); sel-12(ar171); hop-1(RNAi)d,f,g36691(122)
glp-1(ar202)/+h10000(56)
glp-1(ar202)/+; sel-10(ar41)i,e56413(98)
glp-1(ar202)g0973(419)
sup-17(n1258); glp-1(ar202)9910(242)
glp-1(ar202)g0946*(68)
glp-1(ar202); sel-9(ar22)g235918(90)
glp-1(ar224)j0928**(127)
glp-1(ar224); sel-9(ar22)e562024(54)
  • A two-sided Fisher exact test was performed on data marked with an asterisk(s) to test the null hypothesis that the indicated value (“other” vs. Pro + wild type) is different (in either direction) from the corresponding value in the line directly below it. *P value < 0.05; **P value < 0.01.

  • a Actual genotype: glp-1(ar202); unc-1(e719).

  • b “% Pro” includes 5% Pro + Class B. “% other” includes 2% Class A and 2% Class A + Class B.

  • c Actual genotype: glp-1(ar202); sel-12(ar131) unc-1(e719).

  • d Actual genotype: glp-1(ar202); sel-12(ar171) unc-1(e719).

  • e “% other”: Class A + Class B.

  • f The observed level of suppression of glp-1(ar202) by sel-12(ar171); hop-1(RNAi) may be an underestimate since we did not observe a Glp phenotype in sel-12(ar171); hop-1(RNAi) animals scored in a parallel experiment (n = 136). A Glp phenotype was previously observed in sel-12 (ar171); hop-1 (RNAi) animals (Li and Greenwald 1997); however, our RNAi construct differed from that used by Li and Greenwald (1997); see materials and methods.

  • g “% other”: Class A.

  • h Actual genotype: ncl-1(e1865) unc-36(e251) glp-1(ar202)/unc-32(e189); him-5 (e1490). Non-Unc self-progeny were scored from hermaphrodite mothers of the same genotype.

  • i Actual genotype: ncl-1(e1865) unc-36(e251) glp-1(ar202)/unc-32(e189); sel-10(ar41) him-5(e1490). “% Pro” in- cludes 6% Pro + Class B. Non-Unc self-progeny were scored from hermaphrodite mothers of the same genotype. The double-mutant glp-1(ar202); sel-10(ar41) could not be scored due to synthetic lethality.

  • j Actual genotype: dpy-17(e164) glp-1(ar224)/unc-32(e189) glp-1(ar224). “% Pro” includes 78% Pro + Class B. “% other” includes 6% Class A + Class B and 2% Tum.