TABLE 4

Molecular analysis of tagged lines

Analysis categoryT-DNADs transposon
Tagged mutants analyzed3232
No flanking sequence obtaineda50
Insertion in iaaH gene02c
No flanking sequence confirmedb21c
Essential gene not identifiedd114
Essential gene identified1425e
  • a Results from plasmid rescue and/or TAIL-PCR.

  • b Lines were not analyzed further.

  • c Line ET2614 is counted only in the insertion in the iaaH gene category, but it also had another TAIL-PCR sequence that could not be confirmed by PCR.

  • d Lines with possible rearrangements, multigene deletions, and insertions between two genes in which the essential gene was not identified.

  • e Three of the 25 Ds mutant lines disrupt the same gene.