TABLE 1

Rates of mortality in mutagenized lines during inbreeding phase and simulation-based inferences of properties and numbers of mutations lost to selection, assuming EMS mutagenesis induced 20, 45, or 80 mutations

UBackgroundTotal observed
mortality (%)
Selection
coefficient (%)a
Proportion of
mutations
lost (%)a
No. of minor
mutations lost (%)a,b
20Unloaded37.1 (28.5-45.6)1.34 (0.96-1.84)8.2 (5.8-10.6)0.64 (0.16-1.11)
Loaded37.8 (30.2-25.4)1.38 (1.04-2.99)8.4 (6.3-10.5)0.68 (0.26-1.10)
45Unloaded37.1 (28.5-45.6)0.22 (0.15-0.30)8.8 (6.4-11.2)3.0 (1.9-4.1)
Loaded37.8 (30.2-45.4)0.23 (0.17-0.29)9.0 (6.9-11.2)3.1 (2.1-4.0)
80Unloaded37.1 (28.5-45.6)0.066 (0.050-0.089)9.0 (6.5-11.4)6.2 (4.2-8.1)
Loaded37.8 (30.2-45.4)0.068 (0.052-0.088)9.2 (7.0-11.3)6.3 (4.6-8.1)
  • a Obtained by comparison with simulation results.

  • b Assuming a Poisson mean of U EMS-induced mutations of minor effect (s < 0.5) and one of major effect (s > 0.5) per haploid genome.