TABLE 5

Transformation rescue of spe-29 hermaphrodites

ConstructF25H8.2aspe-29anbRescued
linesc
Self-
progenyd
Maximum
progeny
40NA417
pJN115++64780 ± 10121
pJN136+721381 ± 15186
pJN142+1230NANA
  • a + or — refers to the presumed functionality of the indicated gene in each construct. See materials and methods or details on gene disruption and construct synthesis.

  • b n, sample size of F1 transformants (Rol phenotype).

  • c F1 transformants were judged to be rescued if their F2 brood sizes were ≥24 (2 standard deviations greater than the maximum number of progeny observed in untransformed animals) and contained some Rol individuals.

  • d Average self-progeny numbers were calculated only from rescued individuals, except for control injections, where broods from all F1 transformants were averaged to determine the baseline fertility.