TABLE 1

Data summary

Exp.
no.
Relevant
genotype
AntibodyMicroscopeNo. of
nuclei
No. of
embryos
Average
age (cells)
DPY-staining
bodies
per nucleus
% nuclei
with
1 body
% nuclei
with
2 bodies
% nuclei
with
3 bodies
% nuclei
with
4 bodies
Average
nuclear
vol.
(×106 nm3)
Average
DPY-staining
vol.
(×106 nm3)
Average
% nucleus
occupied by
DPY staina
Wild type
1N2DPY-27Zeiss 410100681261.3766.430.03.30.31396413489.91
2N2DPY-27Zeiss 41072051441.4262.633.23.90.315120149310.27
3N2DPY-26Leica144791611.4162.833.03.80.412354165813.99
4N2DPY-26Leica99351991.4961.530.26.61.013254176314.26
TotalN2DPY-26&27Both4166271541.4163.631.64.20.513510bb
mnDp10
1mnDp10DPY-27Zeiss 41080761351.6352.533.512.31.114555146210.45
2mnDp10DPY-27Zeiss 4106717961.5950.840.57.80.916982186711.57
3mnDp10DPY-26Leica1713101711.6649.038.310.42.2123841826
4mnDp10NDNDNDNDNDNDNDNDNDNDNDNDND
TotalmnDp10DPY-26&27Both3191231391.6350.537.710.11.514350bb
yDp14
1yDp14DPY-27Zeiss 41059431981.3963.034.32.70.010297115711.80
2yDp14NDNDNDNDNDNDNDNDNDNDNDNDND
3yDp14DPY-26Leica75541891.3369.727.33.00.08747123414.66
4yDp14DPY-26Leica41031371.464.631.72.40.712745150212.47
TotalyDp14DPY-26&27Both1759101761.3766.130.72.80.210411bb
stDp2
4stDp2DPY-26Leica3134132411.4663.729.05.61.411666156514.68
mnDp27
4mnDp27DPY-26Leica137452751.4465.329.85.20.910233144415.28
  • DPY-27 antibodies were used in experiments 1 and 2, and DPY-26 antibodies were used in experiments 3 and 4. Due to differences between the microscopes and/or the antibodies, volume measurements from experiments 1 and 2 cannot be compared directly with those from experiments 3 and 4. ND, no data.

  • a This number is the average of the percentage of each nucleus occupied by DPY staining, and therefore will not be equal to the average DPY-staining volume/average nuclear volume.

  • b The overall averages for values dependent on DPY staining cannot be calculated because of the different properties of DPY-26 and DPY-27 antibody staining.