TABLE 7

The PKC pathway mediates FUS1-lacZ expression in dpm1-101 cells

GenotypeFUS1-lacZ expressionaAT resistance (mm)b
Wild type301
bck1311
slt2271
ste4<0.2His
ste4 bck1<0.2His
ste4slt2<0.2His
dpm1-101735
dpm1-101 bck1305
dpm1-101 slt2345
dpm1-101 ste4551
dpm1-101 ste4 bck1161
dpm1-101 ste4 slt23His
Wild type (pRS314)281
Wild type (pBCK1-20)10810
  • a β-Galactosidase activity was determined as described in materials and methods. The reported values are the average of at least three independent determinations.

  • b The highest concentration of aminotriazole (AT) tested that allowed growth on synthetic medium lacking histidine.