TABLE 4

Expression of FUS1-lacZ in mutants defective for protein glycosylation

Relevant genotypeaCalcofluorb(μg/ml)FUS1-lacZc
ste4Δ800.37
ste4Δ spt14-2101.6
ste4Δ och1Δ105.7
ste4Δ mnn10Δ101.4
ste4Δ alg1-1101.5
ste4Δ80<0.2
ste4Δ + tunicamycindN/A4.2
ste4Δ + tunicamycin (SD)eN/A0.6
Wild typeN/A30
Wild type + tunicamycinN/A58
  • a Analysis of glycosylation mutants was performed in the W303-1A-derived ste4Δ cells containing an integrated FUS1-lacZ reporter. Cells were incubated at 37° for 5 hr for strains containing the temperature-sensitive alleles alg1-1 and spt14-2. The tunicamycin experiments were performed in the SY2002 background.

  • b The highest concentration of Calcofluor tested that allowed growth on YPD plates.

  • c β-Galactosidase activity was determined as described in materials and methods. The reported values are the average of at least three independent determinations.

  • d Tunicamycin was added at a concentration of 25 μg/ml to log-phase cultures; inductions were for 16 hr in YPD.

  • e Cells were induced in synthetic medium containing glucose.