TABLE 4

Frequency of HIS3:telV loss and retention

DSB-induced recombination frequencies × 104
Ura+Ura-
StrainanbHis+cHis-His+/-His+cHis-His+/-Ura+/-d
JC3517-132436768298106341e2533
JC3518-4314546430512101022
  • a JC3517-13 and JC3518-4 both carry HIS3:telV on the ura3-HO432 chromosome.

  • b Number of colonies analyzed.

  • c ̃15% of His+ products gained a second copy of HIS3:telV; the rest remained heterozygous.

  • d Includes four classes: Ura+/- His+/+, Ura+/- His-/-, Ura+/- His+/-, and Ura+/- His-/+.

  • e About 30% of these nonsectored, Ura- His- products from JC3517-13 arose by chromosome loss; no chromosome loss was detected in JC3518-4.