TABLE 2

Spontaneous and DSB-induced recombination frequencies

Recombination frequency (X104)a
Ura+Ura-Ura-/Ura+ + Ura-):
Gal
Expt.StrainbAssaycndGluGalGluGal
1aSW3516-4Selective42.2 ± 1.8212 ± 115NANANA
1bSW3516-4Nonselective41.8 ± 3.5237 ± 8521 ± 29208 ± 710.52 ± 0.02
2aSW3516-4Selective4ND295 ±111NANANA
2bSW3516-4Nonselective4ND321 ± 44ND318 ± 420.47 ± 0.09
3aDY3515-13Selective45.9 ± 5.1269 ± 90NANANA
3bDY3515-13Nonselective40e402 ± 1226 ± 12843 ± 2970.67 ± 0.04
4aDY3515-13Selective4ND298 ± 160NANANA
4bDY3515-13Nonselective4ND378 ± 84ND753 ± 750.67 ± 0.04
5JC3520-13Selective110.01 ± 0.01NDNANANA
6JC3521-4Selective110.05 ± 0.03NDNANANA
  • NA, not applicable (since Ura- products are not recovered in selective assays); ND, not determined; Expt., experiment.

  • a Recombination frequencies (averages ± SD) are given for glucose (Glu) and galactose (Gal) grown cultures (uninduced and DSB-induced, respectively).

  • b DY3515-13, JC3520-13, SW3516-4, and JC3521-4 have GALHO; JC3520 13, and JC3521-4 lack GALHO.

  • c In selective assays Ura+ recombinants were identified by directly plating on selective medium; in nonselective assays, Ura+ recombinants were identified by replica plating colonies grown initially on YPD medium. In nonselective assays, 1300-11,000 individual colonies were scored per experiment.

  • d Number of independent populations tested.

  • e No Ura+ colonies arose from any of the four populations, totaling 7790 cells.