TABLE 9

Various comparisons of conjugations and transductions

SetTableCross
Backcross
Donor
marker at
NR
(kb)
S
(kb)
IPTIL
(kb)
S/L
A. Transductional crosses and backcrosses
1-47→K12trpA3328min18128812
-47K-4→K12trpA3315232300240.96
-47K-9→K12trpA3315191900191.00
2“R-”7A47→ER247628min20353055
“R-”7B47D10→ER247630323211630.51
B. Conjugational crosses and backcrosses
33A47→K1228min2951161221
3B47K12#12→K1230575700590.97
4“R-”8A47→ER247628min30423133
“R-”8B47KR#15→ER2476159090001000.90
5“R-”-47→C1a28min15484800
“R-”-47C1a#15→C1a15105105001210.87
C. Conjugational reciprocal crosses
64A47→K1218min304111
4BK12→473026020047
75A72→K1218min1534222478
5BK12→721568668600
86A47→7218min30575700
6B72→471536827245
9“R-”-47→C1a18min15461023
C1a471534623161
  • N, number of progeny; R, average length of range of donor DNA; S, average maximum segment length; IP, number of progeny with donor DNA interruptions; TI, total number of interruptions; L, length of original donor DNA segment in backcross donor. Origin of transfer is at 31 min in all cases. In sets 1-5, initial crosses are placed above the line and backcrosses below. “R-” indicates a nominally restrictionless recipient, ER2476 or C1a.