TABLE 5

Distribution of donor and recipient DNA in reciprocal transconjugants

A. Conjugation: DCHF2 (ECOR72) → DCRA33 (K12)
Minutes
90//29121516182022242527----28-----2930
+IPCNS*GDHHPNBEAN
LHSACLMYTOAUDHR
TransVGGNSART#
8+-DDDD*----------(1)
14+--DDD*DD--------
4+--DDD*D---------
3+-----*DD--------
6, 9+---DD*----------
5, 13+-----*----------
1+DDDDD*--D-------(2)
10+-----*D-----DD--
15+-DDD-*D---------
11+-----*D----/d-----
7+-----*-DDD------
12+-----*--D-------
2+DDDDD*-D--DDDDD-(3)
B. Conjugation: K12 trpA33 Hfr → ECOR 72 str
Minutes
75//2912151617182022242527----28-----2930
+IPCNSB*GDHHPNBEAN
LHSACILMYTOAUDHR
TransVGGONSART#
F72- 1, 4, 8, 11+-DDDDD*DDDDDDDDDD(1)
7+-DDDDD*DDDDDDDDD-
5, 6+--DDDD*DDDDDDDDDD
2+DDDDDD*D---------
12+---DDD*DDDDDDDDD-
10+----DD*DDDDDDDDDD
13, 14+-----D*DDDDDDDDDD
3, 9+---DDD*DD--------
15+---DD-*DDD-------(2)
  • +, counterselected marker, rpoB (at 90 min, top), or rpsL (at 75 min, bottom). Other symbols as in Table 2.