TABLE 4

Distribution of donor and recipient DNA in reciprocal transconjugants

Conjugation DCHF4 (ECOR 47) → DCRA33 (K12)
Minutes
7516171820242730
+SB*GDHNN
CILMTAR
TransONSR#
2, 4, 5, 7-30+--*-----(1)
3+--*D----
6+-D*-----
1+--*-D---(2)
Conjugation: DCHF3 (K12) → ECOR 47
Minutes
75291215161718202224252728--------29--30
+IPCNSM*GDHHPNBSETATN
LHSACOLMYTOAUQDAHYR
TransVGGANSARTR#
  4+-----D*DDDDDDDDDDDDD(1)
22+----DD*DDDDDDDD-----
30+-DDDDD*DDDD---------
11, 29+---DDD*DDDD---------
25+-----D*DDDD---------
14+---DDD*DDD----------
  7+-----D*DDD----------
  5+------*DDD----------
17, 21, 28+---DDD*DD-----------
18+-----D*DD-----------
23+-DDDDD*D------------
12, 15+----DD*D------------
19+---DDD*D------------
  3, 13, 10, 20, 26+-----D*D------------
10+-----D*D------------
20+-----D*D------------
26+-----D*D------------
13+-----D*D------------
  9+---DDD*-------------
24+----DD*-------------
  8, 16+-----D*-------------
  1+-----D*D-DDD--------(2)
27+--DDDD*----DDD-D----
  2+----DD*D--D---DDDDDD
  6+---DDD*DDD--DD-DDD--(3)
  • *, selected Tn10 marker near 18 min; +, counterselected marker, rpsL. Other symbols as in Table 2.