TABLE 3

Distribution of donor and recipient DNA in transconjugants

A. Conjugation: ECOR 47 → K12 W3110 trpA33
Minutes
75//202223.52527--28-----------------------------------------------------------------------------2930
kb3111111111111111111111
49011223333333333333333
74318790001111111223368
21354675680124569780525
+DHHPNAGGBOLAS*CEBSTAAT
MYTOADOLUNKLQBDSBACHY
TransSARTR#
12+----DDDDDDDDD*DDDDDD--(1)
4+-----DDDDDDDD*DDDDDD--
8+-----DDDDDDDD*DDDDDD--
11+DDDD-DDDDDDDD*DDDDDDD-
21+-----DDDDDDDD*DDD-----
3+------DDDDDDD*DDDDDD-
7+------DDDDDDD*DDDD----
16+-----------DD*DDDDDD--
13+------------*DDDDDD--
25+-------------*DDDDDD--
29+------DDDDDDD*D-------
22+------DDDDDDD*D-------
6+-------DDDDDD*--------
14+------------D*DD------
24+-------------*DD------
15+-----------DD*D-------
26+-------------*D-------
27+------DDDDDDD*-DDDDD--(2)
2+----D-------D*--------
28+------DD-DDDD*DDD-----
-
17+-------DdDDD*DD------
-
18+--------DdDDD*DD------
1+---------d---D*--------
19+-DD---DDDDDDD*DDDDd-D--(3)
30+DDD-D------DD*D-------
23+------------D*-D---d---
20+---D---------*D-d-d------(4)
10+------DDDDD-D*d--d--D---
9+--DD---DDDD--*D-dDDd-d---(5)
5+------mmmmmmm*mmmmmm--
B. Backcross HAZ-12 (ECOR 47 → K12 W3110 trpA33) → K12
Minutes
90//2527--28------------------------------------------------------------------------------------------------29
+PNAGGBOLAS*CEBSTAA
OADOLUNKLQBDSBACH
TransT#
[Donor HAZ-12:+-DDDDDDDDD*DDDDDD-]
JAZ-1-3, 5-28, 30+-DDDDDDDDD*DDDDDD-(1)
4+-----DDDDD*DDDDDD-
29+---------D*DDDDDD-
  • Symbols as in Table 2. *, selected on minimal medium for trpA+ at 28 min; -d, d-, -d-, d-d, fragment is mosaic as indicated; +, counterselected marker, rpsL (at 75 min, top) or rpoB (at 90 min, bottom); m, mixed donor and recipient bands (not compiled).