TABLE 2

A representative data set. Conjugation: BW7622 → ECOR 47/Hfr origin 47 min

Minutes
75//912161820222527.5----28--------------------------------------------------29--------303336
kb31111111111111111111111
4457890122233333333333333356
7055443879900011111223366938
2249613467956812459780527785
+PCSGDHPNAOGGBLAS*EBSTAAPNNE
HSCLMYOADPOLUKLQDSBACHSRZF
GNSATP
Trans#
6+---DDDDDDDDDDDDD*DDDDDDDx--(1)
22+------DDDDDDDDDD*DDDDDDDxD-
5+-----DDDDDDDDDDD*DDDd-------
20+------DDDDDDDDDD*DDDDDDDx--
8+------DDDDDDDDDD*DDDDDDDx--
23+-------------DDD*DDDDDDDxD-
11+------DDDDDDDDDD*DDDDD-----
12+------DDDDDDDDDD*DDDDD-----
7+------DDDDDDDDDD*DDDDD-----
27+------DDDDDDDDDD*DDDDD-----
2+-------DDDDDDDDD*----------
1+-D-----DDDDDDDDD*DDDDDDDx--(2)
21+--D---DDDDDDDDDD*DDDDDDDx--
25+----DD-DDDDDDDDD*DDDDD-----
4+-------D-DDDDDDD*DDDDDDD---
19+-------DDDDDDDDD*D-DDD-----
26+-------D-----DDD*----------
28+---DD---d--------*DDDDD-----(3)
24+-------D--DD-DDD*-DDd----xD-(5)
  • Transconjugants are arranged in sets in order of number of donor DNA segments and (within these sets) according to range of donor DNA. Trans, transconjugant strain; #, number of donor segments; *, selected marker, Tn10 in trpB; +, counterselected marker, rpsL; d-, fragment contains donor DNA on left and recipient DNA on right. Further symbols are explained in the text. x denotes inability to make the PCR fragment. Hfr BW7622 cannot be amplified with the NR primers used, and all the fragments in NR have the ECOR 47 pattern. Thus, in this cross, it is the donor DNA that evidently cannot be amplified in the NR region.