TABLE 10

Conjugation: DCHf1 → ECOR 47/Hfr origin 31 min

7529101112151618202122242527-28---------------29----------30
+IFPAD*CNSGDMHHPNBETA$APT
LTHCNSACLMUYTOAUDACHSY
TransVSRXGGNSKARTPR#
DK04+------------DDDDDDD$DDD(1)
DK03+--------------DDDDD$DDD
DK07+--------------DDDDD$DDD
DK02+---------------DDDD$DDD
DK01+-----------------/dDD$DDDDK
DK05+-------------------$DDD
DK10+-------------------$DDD
DK06+-------------------$DDD
DK09+--------------D---D$DD-(2)
DK08+-DDD*DDDDDD--------$DDD
DB01+DDDD*DDDDDDDDDDDDDD$DDD(1)
DB05+-DDD*DDDDDDDDDDDDDD$DDD
DB09+----*DDDDDDDDDDDDDD$DDD
DB10+----*DDDDDDDDDDDDDD$DDD
DB04+----*DDD-----DDDDDD$DDD(2)DB
DB08+--DD*D------DDDDDDD$DDD
DB03+--DD*D------DDDDDDD$DDD
DB02+----*D---DDDDDDDDDD$DDD
DB07+--DD*DDD-D----DDDDD$DDD(3)
DB06+-DDD*D-----/dDD----DD$DDD
DT05+-DDD*DDDDDDDDD---------(1)
DT08+----*DDDDD-------------
DT06+----*DDDD--------------
DT03+---D*D-----------------
DT09+----*D-----------------DT
DT02+----*------------------
DT04+-DDD*DDDD--DDDDDDDD$DDD(2)
DT01+----*D--D--------------
DT07+----*D-DDDDD-----------
DT10+--DD*DDD-DD--D----D----(4)
  • The DK strains were selected on kanamycin only; DB on both kanamycin and tetracycline; DT on tetracycline only. Subsequent screening revealed tetracycline resistance in exconjugant DK-08 and kanamycin resistance in DT-04. Trans, transconjugant strain; #, number of donor segments; *, tetR donor marker; $, kanR donor marker; +, recipient marker, rpsL. Other symbols as in Table 2.