TABLE 4

A comparison of the sequence similarities between genes with known mutant phenotypes and those without

P valueOther speciesVertebratesC. elegansS. cerevisiaePlantsBacteriaDrosophilaAll
% of the 218 predicted genes in the Adh region with BLAST scores better than the indicated P value when compared to the indicated subsets of GenBanka
<e-766 (51)57 (51)55 (53)31 (66)24 (68)30 (51)47 (48)71 (48)
<e-2051 (55)45 (55)37 (55)19 (78)17 (64)12 (60)36 (51)58 (53)
<e-5031 (60)27 (64)18 (67)9 (80)8 (82)3 (50)25 (63)41 (60)
<e-10014 (87)13 (93)8 (83)3 (100)3 (83)017 (81)23 (80)
% of 49 genes known to display loss-of-function phenotypes, with BLAST scores better than the indicated P value when compared to the indicated subsets of GenBanka
<e-790 (80)84 (78)76 (78)55 (81)39 (84)43 (71)78 (71)94 (80)
<e-2082 (80)76 (78)64 (77)37 (100)27 (85)18 (89)76 (70)94 (80)
<e-5063 (77)61 (77)37 (94)22 (100)14 (100)2 (100)67 (73)84 (77)
<e-10037 (100)37 (100)20 (100)10 (100)6 (100)653 (81)65 (84)
% of 145 genes predicted to lack loss-of-function phenotypes, with BLAST scores better than the indicated P value when compared to the indicated subsets of GenBanka
<e-754 (27)44 (25)45 (32)19 (41)17 (48)23 (32)32 (20)59 (26)
<e-2035 (25)29 (24)23 (24)10 (36)12 (41)10 (29)17 (4)40 (26)
<e-5014 (19)9 (23)8 (8)3 (0)5 (57)3 (40)3 (0)19 (25)
<e-1002 (0)02 (0)01 (50)002 (33)

To calculate the expected percentage of the 145 genes that did not have loss-of-function phenotypes (218 total genes—73 with such phenotypes) we made the assumption that the 24 genes with phenotypes that we were unable to assign to a specific open reading frame (ORF; 73 genes with loss-of-function phenotypes—49 such genes assigned to an ORF) had the same probability of having a BLAST hit at a particular P value, and the same probability of having an EST match, as the 49 genes we could assign to single ORFs. We multiplied the number of the 49 genes with a phenotype that had a BLAST hit at a particular value of P or an EST match by 73/49 and then subtracted this number from the corresponding number derived using 218 genes in the Adh region.

  • a The percentage of these genes that also have EST matches is given in parentheses.