TABLE 1

Strains and their genotypes

StrainGenotypeSourcea
BP95mat3-P(EcoRV)::ura4 ura4-D18 ade6-delXB clr2-E22This study
BP100mat3-M(EcoRV)::ade6ori1 ura4-D18 ade6-delXB clr2-E22BP95 TW pPB17b
BP141mat3-M(EcoRV)::ade6ori1 leu1-32 ura4-D18 ade6-M210This study
BP181mat3-M(EcoRV)::ade6ori1 ura4-D18 ade6-M210 swi6-115This study
PG1leu1-32 ura4-D18 ade6-M216 swi6-115Thon and Friis (1997)
PG9mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216Thon and Klar (1992)
PG355mat3-P(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M210This study
PG445mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M216This study
PG447mat1-PΔ17::LEU2 mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216Thon and Klar (1992)
PG587Δ(H3)mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M210PL12 × SP980
PG1049mat1-PΔ17::LEU2 mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115This study
PG1063mat1-Msmt-0 mat2-P(XbaI)::ura4 ura4-D18 ade6-M210 swi6-115 esp3-1Thon and Friis (1997)
PG1141leu1-32 ura4-D18 ade6-M210This study
PG1165mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 leu1-32 ura4-D18 ade6-M216 esp3-1This study
PG1174mat1-Msmt-0 mat2-P(XbaI)::ura4 ura4-D18 ade6-M216 esp3-1This study
PG1192mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT79d482c
PG1193mat1-PΔ17::LEU2 Δ(1185)mat3-Mleu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT79d1185c
PG1194mat1-PΔ17::LEU2 mat3-MΔ(424) leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT70d424c
PG1195mat1-PA 17::LEU2 Δ(1510)mat3-MΔ (424) leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT181c
PG1357mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M210This study
PG1397mat1-PA 17::LEU2 Δ(1510)mat3-MΔ (424) leu1-32 ura4-D18 ade6-M210PG1195 × PG1357
PG1398mat1-PΔ17::LEU2 Δ(1185)mat3-Mleu1-32 ura4-D18 ade6-M210PG1193 × PG1357
PG1399mat1-PΔ17::LEU2 Δ(1185)mat3-Mleu1-32 ura4-D18 ade6-M210 swi6-115PG1193 × PG1357
PG1401mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210 swi6-115PG1192 × PG1357
PG1403mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210PG1192 × PG1357
PG1404mat1-PΔ17::LEU2 mat3-MΔ(424) leu1-32 ura4-D18 ade6-M210PG1194 × PG1357
PG1412mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210 clr1-5This study
PG1418mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210 clr3-735This study
PG1420mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210 clr4-681This study
PG1421mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M210 clr2-760This study
PG1435mat1-PΔ17::LEU2 Δ(482)mat3-Mleu1-32 ura4-D18 ade6-M216 esp3-1PG1174 × PG1403
PG1526mat1-PΔ17::LEU2 Δ(1185)::(424)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT142c
PG1527mat1-PΔ17::LEU2 Δ(482)::(424)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT132c
PG1529mat1-PΔ17::LEU2 Δ(482)::(424)oriIImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT133c
PG1531mat1-PΔ17::LEU2 Δ(482)::(134)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT124c
PG1534mat1-PΔ17::LEU2 Δ(482)::(136)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT125c
PG1538mat1-PΔ17::LEU2 Δ(482)::(88)oriImat3-Mleu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT126c
PG1541mat1-PΔ17::LEU2 Δ(482)::(126)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT127c
PG1548mat1-PΔ17::LEU2 Δ(482)mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1192 TW pGT77d
PG1550mat1-PA 17::LEU2 Δ(482)::mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216SP1125 × PG1548
PG1554mat1-PΔ17::LEU2Δ(482)::(126)oriIImat3-Mleu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT131c
PG1557mat1-PΔ17::LEU2 Δ (1185)::(126)oriImat3-M leu1-32 ura4-D18 ade6-M216 swi6-115PG1049 TW pGT137c
PG1560mat1-PΔ 17::LEU2 Δ(H3)mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M210PG587 × PG445
PG1562mat1-Msmt-0 mat2-P(XbaI)::ura4 mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115BP181 × SP1126
PG1564mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115BP181 × SP1138
PG1566mat1-Msmt-0 leu1-32This study
PG1570leu1-32 ura4-D18This study
PG1579mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M210 clr3-735This study
PG1582mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M210 clr2-760This study
PG1584mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M210 swi6-115This study
PG1587mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M210 clr1-5This study
PG1594mat1-PΔ17::LEU2 leu1-32 ura4-D18 ade6-M216 clr4-681This study
PG1595mat1-Msmt-0 mat2-P(XbaI)::ura4 mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1562 × SP1122
PG1596mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1564 × SP1122
PG1610Δ(H3)mat3-M(EcoRV)::ade6ori1 leu1-32 ura4-D18 ade6-M210 swi6-115PL1 TW pPB17e
PG1612mat1-PΔ17::LEU2 Δ(482)mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210 swi6-115PG1041 TW pPB17e
PG1614mat1-PΔ17::LEU2 Δ(H3)mat3-M(EcoRV)::ade6ori1 leu1-32 ura4-D18 ade6-M210 swi6-115PG1399 TW pPB17e
PG1615mat1-PΔ17::LEU2 Δ(H3)mat3-Mleu1-32 ura4-D18 ade6-M210 swi6-115PL1 × PG1049
PG1616mat1-PΔ17::LEU2 Δ(1185)::(424)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1526 TW pGT77d
PG1617mat1-PΔ17::LEU2 Δ(482)::(424)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1527 TW pGT77d
PG1619mat1-PΔ17::LEU2 Δ(482)::(424)oriIImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1529 TW pGT77d
PG1620mat1-PΔ17::LEU2 Δ(482)::(126)oriIImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1554 TW pGT77d
PG1622mat1-PΔ17::LEU2 Δ(482)::(126)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1541 TW pGT77d
PG1624mat1-PΔ17::LEU2 Δ(1185)::(424)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M210PG1616 × SP1125
PG1626mat1-PΔ17::LEU2 Δ(482)::(424)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216PG1617 × SP1125
PG1629mat1-PΔ 17::LEU2 Δ(482)::(424)oriIImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216PG1619 × SP1125
PG1630mat1-PΔ 17::LEU2 Δ(482)::(126)oriIImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216PG1620 × SP1125
PG1632mat1-PΔ17::LEU2 Δ(482)::(126)oriImat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216PG1622 × SP1125
PG1646mat1-PΔ17::LEU2 mat2-P(XbaI)::ura4 leu1-32 ura4-D18 ade6-M210This study
PG1647Δ(H3)mat3-M(EcoRV)::ade6ori1 leu1-32 ura4-D18 ade6-M210PG1610 × SP1125
PG1649mat1-PΔ 17::LEU2 Δ(482)mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210PG1612 × SP1125
PG1650mat1-PΔ17::LEU2 Δ(1185)mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210PG1614 × SP1125
PG1654mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(H3)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1610 × SP1126
PG1655mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(482)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1612 × SP1126
PG1656mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(1185)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1614 × SP1126
PG1657mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(H3)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1610 × SP1138
PG1658mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(482)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1612 × SP1138
PG1659mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(1185)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210 swi6-115PG1614 × SP1138
PG1671mat1-PΔ 17::LEU2 Δ(H3)mat3-M(Eco RV)::ade6ori1 leu1-32 ura4-D18 ade6-M210PG1647 × PG1646
PG1672mat1-PΔ 17::LEU2 mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210BP141 × PG1646
PG1675mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(482)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1655 × PG1646
PG1680mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(H3)mat3-M(Eco RV)::ade6oriI ura4-D18 ade6-M210PG1657 × PG1646
PG1681Δ(482)mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210PG1649 × PG1141
PG1682Δ(1185)mat3-M(EcoRV)::ade6oriI leu1-32 ura4-D18 ade6-M210PG1650 × PG1141
PG1683mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(482)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1658 × PG1646
PG1685mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 Δ(1185)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1659 × PG1646
PG1686mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(H3)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1654 × PG1646
PG1688mat1-Msmt-0 mat2-P(XbaI)::ura4 Δ(1185)mat3-M(EcoRV)::ade6oriI ura4-D18 ade6-M210PG1656 × PG1646
PG1690mat1-PΔ 17::LEU2 Δ(H3)mat3-M leu1-32 ura4-D18 ade6-M210This study
PG1741mat1-PΔ 17::LEU2 leu1-32 ura4-D18 ade6-M210 swi6-115 esp3-1PG445 × PG1063
PG1742mat1-PΔ 17::LEU2 leu1-32 ura4-D18 ade6-M210 esp3-1PG445 × PG1063
PL1Δ(H3)mat3-Mleu1-32 ura4-D18 ade6-M210 swi6-115PG355 TW pGT79c
PL6Δ(H3)mat3-Mleu1-32 ura4-D18 ade6-M216PL1 × PG9
PL12Δ(H3)mat3-M(EcoRV)::ura4 leu1-32 ura4-D18 ade6-M216 swi6-115PG1 TW pGT180c
SP980his2 ade6-M210This study
SP1122mat1-PΔ 17::LEU2 mat2-P(XbaI)::ura4 leu1-32 ura4-D18 ade6-M216This study
SP1124mat1-Msmt-0 mat2-P(XbaI)::ura4 ura4-D18 ade6-M216Thon et al. (1994)
SP1125mat1-Msmt-0 mat2-P(XbaI)::ura4 ura4-D18 ade6-M210This study
SP1126mat1-Msmt-0 mat2-P(XbaI)::ura4 ura4-D18 ade6-M210 swi6-115Thon et al. (1994)
SP1138mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 ura4-D18 ade6-M210 swi6-115Thon et al. (1994)
SP1151mat1-Msmt-0 Δ(BglII-BssHII)mat2-P(XbaI)::ura4 ura4-D18 ade6-M216Thon et al. (1994)
  • a Strains were constructed by transformation with restricted plasmid DNA (TW), by genetic crosses of strains listed here (×) or by crosses involving strains not listed here (This study), in which case the complete genealogy is available upon request. Mutated and engineered alleles used for the strain constructions studies: mat1-PΔ17::LEU2: Arcangioli and Klar (1991); mat1-Msmt-0: Engelke et al. (1987) and Styrkarsdottir et al. (1993); mat3-M(EcoRV)::ura4: Thon and Klar (1992) mat3-P(EcoRV)::ura4: Thon and Klar (1993) mat2-P(XbaI)::ura4: Thon et al. (1994); ura4-D18: Grimm et al. (1988); ade6-M210 and ade6-M216: Gutz (1963); ade6-delXB: Zahn-Zabal et al. (1995); swi6-115: Gutz and Schmidt (1985); clr1-5: Thon and Klar (1992); clr2-E22: Ekwall and Ruusala (1994); clr2-760, clr3-735, clr4-681: Thon et al. (1994).

  • b Restricted with PstI and SalI.

  • c Restricted with HindIII.

  • d Restricted with PstI and MspAI.

  • e Restricted with EcoRI.