TABLE 2

Effect on viability and/or fertility of two elav transgenes

Progeny
ParentsFemales carrying two transgenesMales carrying two transgenesFemales carrying zero or one transgeneMales carrying zero or one transgeneSegregation of flies with two transgenesFertile femalesFertile males
Cross no.FemalesMales
1TfS-1 TfL 112 (113)122 (113)110 (113)110 (113)Expected1010
2 TfSm 81 (82)81 (82)83 (82)84 (82)Expected1010
StockTfS-10 (50)0 (50)150 (100)150 (100)AberrantNANA
3TfS-225 (29)14 (29)92 (88)104 (88)Aberrant24
4TfS-2bis35 (52)28 (52)136 (104)112 (104)Aberrant12
5TfS-335 (31)26 (31)61 (62)64 (62)Expected010
6TfS-2 TfL 79 (79)75 (79)82 (79)79 (79)Expected89
7 TfSm 88 (88)87 (88)93 (88)84 (88)Expected1010
8TfS-139 (47)32 (47)158 (141)146 (141)Aberrant38
StockTfS-20 (56)0 (56)167 (111)167 (111)Aberrant00
9TfS-2bis0 (31)0 (31)107 (61)77 (61)Aberrant00
10TfS-344 (54)17 (54)186 (163)189 (163)Aberrant21
11TfS-2bis TfL 34 (35)37 (35)39 (35)32 (35)Expected1010
12( elavnull background) TfSm 79 (75)78 (75)69 (75)0 (0)Expected410
13TfS-131 (42)35 (42)133 (126)95 (84)Aberrant010
14TfS-215 (33)5 (33)78 (65)98 (65)Aberrant00
StockTfS-2bis18 (30)8 (30)77 (61)80 (61)Aberrant00
15TfS-327 (22)19 (22)73 (66)34 (44)Expected00
16TfS-3 TfL 95 (89)85 (89)80 (89)98 (89)Expected1010
17 TfSm 88 (88)87 (88)93 (88)84 (88)Expected1010
18TfS-141 (43)39 (43)80 (86)99 (86)Expected15
19TfS-251 (47)2 (47)171 (142)154 (142)Aberrant00
20TfS-2bis59 (40)0 (40)80 (80)102 (80)Aberrant00
StockTfS-33 (60)0 (60)180 (121)180 (121)Aberrant00

Tf S-1 stock: y elav+ w/ y elav+ w/Y; Tf(2)DmORF3/CyO. TfS-2 stock: y elav+ w/y elav+ w/ Y;Tf(3)DmORF2/TM3Sb. TfS-2bis stock (same insert as in TfS-2): elav e5 w sn/ elav e5 w sn/ Y; Tf(3)DmORF2/TM3Sb. TfS-3 stock: y elav+ w/ y elav+ w/ Y; 339-17/CyO. TfL stock: y elav+ w/ y elav+ w/Y; 350-83-1/350-83-1. TfSm stock; y elav+ w/ y elav+ w/ Y; FliJ1- 7/FliJ1-7. See Figure 1 and Table 1 for the detailed properties of the transgenes. For each category of progeny, the numbers of flies observed are given; numbers of flies expected are given in parentheses. Expected numbers for each class of progeny were calculated as a fraction (predicted by Mendelian rules) of the total progeny from a cross. Chi-square values were calculated to test the hypothesis that flies carrying two transgenes were obtained at expected rates (P < 0.05). The number of fertile flies among the 10 tested individuals carrying two transgenes is indicated (when <10 of those flies were obtained, all were tested). NA, not applicable.