TABLE 3

Dosage studies with n1761 and n1790

GenotypePercentage dead larvaePercentage abnormal vulvaPercentage Muvn
+/+000200
n1761/nulla10101490
n1761/+b000553
n1761/n176173319359
n1761/n1761/+cNDd160183
smg-1; n1761/n1761e64288181
n1790/nullf01275139
n1790/+g00067
n1790/n1790175411285
n1790/n1790/+hNDd131163
smg-1; n1790/n1790e438112168
smg-1eND00303
  • For homozygous strains, we placed each egg on a separate Petri dish and observed development. Heterozygous animals were generated in mating experiments using marker mutations to distinguish self-progeny from cross-progeny. Columns are as defined in Tables 1 and 2.

  • a These n1761/lin-1(sy254) unc-24 hermaphrodites were non-uncoordinated (non-Unc) cross-progeny of n1761 males and lin-1(sy254) unc-24/nT1 n754 hermaphrodites. The nT1 n754 chromosome causes a recessive lethal and dominant Unc phenotype.

  • b These hermaphrodites were Unc non-Dpy cross-progeny of wild-type males and n1761; dpy-11 unc-41 hermaphrodites.

  • c These n1761 dpy-13; mDp1 hermaphrodites were non-Dpy self-progeny of hermaphrodites with the same genotype. mDp1 contains lin-1(+) and dpy-13(+).

  • d ND, not determined. We could not reliably determine the percent dead larvae of animals of this genotype, because mDp1 is not transmitted to every progeny animal, and mDp1 rescue of the Dpy-13 phenotype cannot be scored in dead larvae.

  • e These strains also contained unc-54(r293).

  • f These animals were genotype n1790/lin-1(sy254) unc-24; we picked non-Unc cross-progeny of n1790/+ males and lin-1(sy254) unc-24/nT1 n754 hermaphrodites, scored their phenotype, and confirmed their genotype by examining self-progeny.

  • g These hermaphrodites were Unc non-Dpy cross-progeny of wild-type males and n1790; dpy-11 unc-41 hermaphrodites.

  • h These n1790 dpy-13; mDp1 hermaphrodites were non-Dpy self-progeny of hermaphrodites of the same genotype.