TABLE 2

Growth phenotypes associated with suppressors of G680V

MutantSingleDouble with G680V
G680V+
P128Q+ ++ + +
L176F+ + + ++ + + +
I177M+ ++ + +
G182C+ + + ++ + +
T189I++ + + +
V192F++ + + +
L216F++ + + +
G240V+ ++ + +
L453F++ + +
E476Q+ + + ++ + + +
N483S++ + + +
L638F+ ++ + + +
• Growth phenotypes of suppressors with and without the G680V mutation. Plaque expansion rates of Dictyostelium SPERA cells transformed with single- or double-mutant myosins are shown. Data are generated by comparing the plaque expansion rate to that of SPERA cells transformed with wild-type myosin. To accurately represent the precision of the data, it is shown in 20% increments, where + represents 20-40% wild-type rate (untransformed SPERA cells fall into this category), ++ is 41-60%, + + + is 61-80%, and + + + + is 80100%.