TABLE 1

Yeast strains and plasmids

Strains and plasmidsGenotypes
MAY1715MATa his3 leu2 ura3 ade2 can1 cdc20-1
MAY2096MATα his3 leu2 ura3 ade2 cdc16-1
MAY2422MATa his3 leu2 ura3 lys2 cyh2
MAY2777MATα his3 leu2 ura3 ade2 trp1 CDC20-50 cyh2 cin8::HIS3 (pMA1208)
MAY2830MATa his3 leu2 ura3 ade2 trp1 cyh2 cin8::HIS3 (pMA1208)
MAY2831MATa his3 leu2 ura3 lys2 trp1 cyh2 cin8::HIS3 (pMA1208)
MAY3150MATa his3 leu2 ura3 ade2 trp1 bub2-71 cyh2 cin8::HIS3 (pMA1208)
MAY4366MATa his3 leu2 ura3 ade2 lys2 mad1::HIS3 cyh2 cin8::LEU2 (pMA1125)
MAY4403MATα his3 leu2 ura3 lys2 cdc20-50 cyh2 cin8::LEU2 (pMA1125)
MAY4438MATa his3 leu2 ura3 ade2 trp1 can1 ura3:PGAL → MPS1-myc
MAY5197MATa his3 ura3 lys2 cyh2 LEU2:PGAL → PDS1
MAY5198MATa his3 ura3 lys2 cyh2 LEU2:PGAL → PDS1-mdb
MAY5331MATa his3 leu2 ura3 ade2 trp1 CDC20-50 cyh2 cin8::HIS3 (pMA1208)
p415-MET25PMET LEU2 CEN
p416-MET25PMET URA3 CEN
pES25CDC20-50 URA3 CEN
pES26CDC20 URA3 CEN
pES33PMETCDC20-50 URA3 CEN
pES34PMETCDC20 URA3 CEN
pES36PMETCDC20 LEU2 CEN
pES37cdc20-1 URA3 CEN
pES39PMETcdc20-1, 50 URA3 CEN
pES40PMETcdc20-1 URA3 CEN
pMA1125CIN8 URA3 CEN
pMA1208CIN8 CYH2 LEU2 CEN
pOC69LEU2:PGALPDS1 integrating vector
pOC70LEU2:PGALpds1-mdb integrating vector
pRS316URA3 CEN