TABLE 4

Allelic recombination in gsg1, mum2 and mum3 mutants

StrainRelevant genotypeHis prototrophs (X104)Leu prototrophs (X105)
MitoticaMeiototicbMitoticaMeiototicb
Y315GSG1, MUM3.4 ± 1.3 (1.0)84.0 ± 15 (1.0)1.4 ± 0.7 (1.0)690.0 ± 110 (1.0)
Y316gsg1::LYS22.1 ± 0.9 (0.6)34.0 ± 11 (0.4)1.5 ± 0.8 (1.1)200.0 ± 71 (0.3)
Y792mum2::LYS20.8 ± 0.3 (0.2)4.8 ± 1.4 (0.06)0.4 ± 0.1 (0.3)1.7 ± 1.2 (0.002)
Y1004mum3::LYS22.7 ± 1.2 (0.8)65.0 ± 14 (0.8)1.1 ± 0.6 (0.8)610.0 ± 110 (0.9)
  • His and Leu prototrophs were selected in diploids carrying heteroalleles at the HIS4 and LEU2 loci.

  • a Mitotic recombination frequencies are median frequencies ± SD derived from three independent cultures for strains Y316 and Y1004 and nine independent cultures from strains Y315 and Y792. Numbers in parentheses are the mitotic frequency normalized to the wild-type strain, Y315.

  • b The meiotic frequency represents the mean frequency from three independent cultures plated after 15 hr in sporulation medium. Numbers in parentheses are the meiototic frequency normalized to the wild-type strain, Y315. Y316, Y792, and Y1004 are transformants of Y315.