TABLE 3

Epistasis analysis

StrainRelevant genotypePercent meiotic divisionsaPercent sporulationbPercent spore viabilityc
Y315GSG1, MUM718797
Y344spo11::TRP160521
Y686spo13::URA3625675
Y316gsg1::LYS223471
Y389gsg1::LYS2 spo11::TRP1253<1
Y896gsg1::LYS2 spo13::URA37235
Y690mum2::LEU2<0.1<0.1NA
Y692mum2::LEU2 spo11::TRP1<0.1<0.1NA
Y691mum2::LEU2 spo13::URA3<0.1<0.1NA
Y1004mum3::LEU2-76550732
Y1007mum3::LEU2-765 spo11::TRP1438<1
Y1010mum3::LEU2-765 spo13::URA3446.524
Y1047MUM4 SPO13576992
Y1048mum4-1 SPO13484810
Y1079MUM4 spo13::URA3296883
Y1080mum4-1 spo13::URA3417371
  • a Percent meiotic division includes bi-, tri-, and tetranucleated cells and was determined by fixing cells after 20 hr in sporulation medium, staining with DAPI, and examining by fluorescence microscopy. A minimum of 300 cells were examined.

  • b Percent sporulation was determined by phase-contrast microscopy; at least 300 cells were counted.

  • c Percent spore viability was determined by dissecting a minimum of 100 spores. NA = not applicable.