Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
- Received May 9, 2013.
- Accepted May 23, 2013.
- Copyright © 2013, The Genetics Society of America