Haploidization in Saccharomyces cerevisiae Induced by a Deficiency in Homologous Recombination
Wei Song, Thomas D. Petes

Abstract

Diploid Saccharomyces cerevisiae strains lacking the RAD52 gene required for homologous recombination have a very high rate of chromosome loss. Two of four isolates subcultured about 20 times (approximately 500 cell divisions) became haploid. These strains were capable of mating with wild-type haploids to produce diploid progeny capable of undergoing meiosis to produce four viable spores.

  • Received December 22, 2011.
  • Accepted February 7, 2012.