Skip to main content
  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus
  • Other GSA Resources
    • Genetics Society of America
    • G3: Genes | Genomes | Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org
  • Log in
Genetics

Main menu

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • Centennial
    • Genetics of Immunity
    • Genetics of Sex
    • Genomic Selection
    • Multiparental Populations
    • FlyBook
    • WormBook
    • YeastBook
  • ARTICLE TYPES
    • About Article Types
    • Commentaries
    • Editorials
    • GSA Honors and Awards
    • Methods, Technology & Resources
    • Perspectives
    • Primers
    • Reviews
    • Toolbox Reviews
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Why subscribe?
    • For institutions
    • For individuals
    • Email alerts
    • RSS feeds
  • Other GSA Resources
    • Genetics Society of America
    • G3: Genes | Genomes | Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org

User menu

Search

  • Advanced search
Genetics

Advanced Search

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • Centennial
    • Genetics of Immunity
    • Genetics of Sex
    • Genomic Selection
    • Multiparental Populations
    • FlyBook
    • WormBook
    • YeastBook
  • ARTICLE TYPES
    • About Article Types
    • Commentaries
    • Editorials
    • GSA Honors and Awards
    • Methods, Technology & Resources
    • Perspectives
    • Primers
    • Reviews
    • Toolbox Reviews
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Why subscribe?
    • For institutions
    • For individuals
    • Email alerts
    • RSS feeds
Previous ArticleNext Article

A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair in Saccharomyces cerevisiae

Anna L. Boudoures, Jacob J. Pfeil, Elizabeth M. Steenkiste, Rachel A. Hoffman, Elizabeth A. Bailey, Sara E. Wilkes, Sarah K. Higdon and View ORCID ProfileJeffrey S. Thompson
Genetics July 1, 2017 vol. 206 no. 3 1389-1402; https://doi.org/10.1534/genetics.116.195735
Anna L. Boudoures
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacob J. Pfeil
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth M. Steenkiste
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel A. Hoffman
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth A. Bailey
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sara E. Wilkes
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah K. Higdon
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeffrey S. Thompson
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeffrey S. Thompson
  • For correspondence: thompsonjs@denison.edu
  • Article
  • Figures & Data
  • Info & Metrics
Loading

Abstract

Histone post-translational modifications play vital roles in a variety of nuclear processes, including DNA repair. It has been previously shown that histone H3K79 methylation is important for the cellular response to DNA damage caused by ultraviolet (UV) radiation, with evidence that specific methylation states play distinct roles in UV repair. Here, we report that H3K79 methylation is reduced in response to UV exposure in Saccharomyces cerevisiae. This reduction is specific to the dimethylated state, as trimethylation levels are minimally altered by UV exposure. Inhibition of this reduction has a deleterious effect on UV-induced sister chromatid exchange, suggesting that H3K79 dimethylation levels play a regulatory role in UV repair. Further evidence implicates an additional role for H3K79 dimethylation levels in error-free translesion synthesis, but not in UV-induced G1/S checkpoint activation or double-stranded break repair. Additionally, we find that H3K79 dimethylation levels are influenced by acetylatable lysines on the histone H4 N-terminal tail, which are hyperacetylated in response to UV exposure. Preclusion of H4 acetylation prevents UV-induced reduction of H3K79 dimethylation, and similarly has a negative effect on UV-induced sister chromatid exchange. These results point to the existence of a novel histone crosstalk pathway that is important for the regulation of UV-induced DNA damage repair.

  • histone modification
  • DNA repair
  • methylation
  • sister chromatid exchange
  • histone crosstalk
  • Received September 8, 2016.
  • Accepted May 16, 2017.
  • Copyright © 2017 by the Genetics Society of America
View Full Text

Log in using your username and password

Forgot your user name or password?
Previous ArticleNext Article
Back to top

PUBLICATION INFORMATION

Volume 206 Issue 3, July 2017

Genetics: 206 (3)

ARTICLE CLASSIFICATION

INVESTIGATIONS
Genome integrity and transmission
View this article with LENS
Email

Thank you for sharing this Genetics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair in Saccharomyces cerevisiae
(Your Name) has forwarded a page to you from Genetics
(Your Name) thought you would be interested in this article in Genetics.
Print
Alerts
Enter your email below to set up alert notifications for new article, or to manage your existing alerts.
SIGN UP OR SIGN IN WITH YOUR EMAIL
View PDF
Share

A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair in Saccharomyces cerevisiae

Anna L. Boudoures, Jacob J. Pfeil, Elizabeth M. Steenkiste, Rachel A. Hoffman, Elizabeth A. Bailey, Sara E. Wilkes, Sarah K. Higdon and View ORCID ProfileJeffrey S. Thompson
Genetics July 1, 2017 vol. 206 no. 3 1389-1402; https://doi.org/10.1534/genetics.116.195735
Anna L. Boudoures
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacob J. Pfeil
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth M. Steenkiste
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel A. Hoffman
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth A. Bailey
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sara E. Wilkes
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah K. Higdon
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeffrey S. Thompson
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeffrey S. Thompson
  • For correspondence: thompsonjs@denison.edu
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation

A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair in Saccharomyces cerevisiae

Anna L. Boudoures, Jacob J. Pfeil, Elizabeth M. Steenkiste, Rachel A. Hoffman, Elizabeth A. Bailey, Sara E. Wilkes, Sarah K. Higdon and View ORCID ProfileJeffrey S. Thompson
Genetics July 1, 2017 vol. 206 no. 3 1389-1402; https://doi.org/10.1534/genetics.116.195735
Anna L. Boudoures
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacob J. Pfeil
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth M. Steenkiste
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel A. Hoffman
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth A. Bailey
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sara E. Wilkes
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah K. Higdon
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeffrey S. Thompson
Department of Biology, Denison University, Granville, Ohio 43023
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeffrey S. Thompson
  • For correspondence: thompsonjs@denison.edu

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Related Articles

Cited By

More in this TOC Section

Investigations

  • Mutational Pleiotropy and the Strength of Stabilizing Selection Within and Between Functional Modules of Gene Expression
  • Regulation of Glutamate Signaling in the Sensorimotor Circuit by CASY-1A/Calsyntenin in Caenorhabditis elegans
  • A Damage Sensor Associated with the Cuticle Coordinates Three Core Environmental Stress Responses in Caenorhabditis elegans
Show more 3

Genome Integrity and Transmission

  • Plasticity of Meiotic Recombination Rates in Response to Temperature in Arabidopsis
  • Mitotic and Meiotic Functions for the SUMOylation Pathway in the Caenorhabditis elegans Germline
  • Selection-Enhanced Mutagenesis of lac Genes Is Due to Their Coamplification with dinB Encoding an Error-Prone DNA Polymerase
Show more 3
  • Top
  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Info & Metrics

GSA

The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.

Online ISSN: 1943-2631

  • For Authors
  • For Reviewers
  • For Subscribers
  • Submit a Manuscript
  • Editorial Board
  • Press Releases

SPPA Logo

GET CONNECTED

RSS  Subscribe with RSS.

email  Subscribe via email. Sign up to receive alert notifications of new articles.

  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus

Copyright © 2018 by the Genetics Society of America

  • About GENETICS
  • Terms of use
  • Advertising
  • Permissions
  • Contact us
  • International access