Large-Scale Mapping of Transposable Element Insertion Sites Using Digital Encoding of Sample Identity
Daryl M. Gohl, Limor Freifeld, Marion Silies, Jennifer J. Hwa, Mark Horowitz and Thomas R. Clandinin
Genetics March 1, 2014 vol. 196 no. 3 615-623; https://doi.org/10.1534/genetics.113.159483
Daryl M. Gohl
Department of Neurobiology, Stanford University, Stanford, California 94305
Limor Freifeld
Department of Electrical Engineering, Stanford University, Stanford, California 94305Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Marion Silies
Department of Neurobiology, Stanford University, Stanford, California 94305
Jennifer J. Hwa
Department of Neurobiology, Stanford University, Stanford, California 94305
Mark Horowitz
Department of Electrical Engineering, Stanford University, Stanford, California 94305
Thomas R. Clandinin
Department of Neurobiology, Stanford University, Stanford, California 94305

Abstract
Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.
- Received November 8, 2013.
- Accepted December 21, 2013.
- Copyright © 2014 by the Genetics Society of America
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Large-Scale Mapping of Transposable Element Insertion Sites Using Digital Encoding of Sample Identity
Daryl M. Gohl, Limor Freifeld, Marion Silies, Jennifer J. Hwa, Mark Horowitz and Thomas R. Clandinin
Genetics March 1, 2014 vol. 196 no. 3 615-623; https://doi.org/10.1534/genetics.113.159483
Daryl M. Gohl
Department of Neurobiology, Stanford University, Stanford, California 94305
Limor Freifeld
Department of Electrical Engineering, Stanford University, Stanford, California 94305Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Marion Silies
Department of Neurobiology, Stanford University, Stanford, California 94305
Jennifer J. Hwa
Department of Neurobiology, Stanford University, Stanford, California 94305
Mark Horowitz
Department of Electrical Engineering, Stanford University, Stanford, California 94305
Thomas R. Clandinin
Department of Neurobiology, Stanford University, Stanford, California 94305
Large-Scale Mapping of Transposable Element Insertion Sites Using Digital Encoding of Sample Identity
Daryl M. Gohl, Limor Freifeld, Marion Silies, Jennifer J. Hwa, Mark Horowitz and Thomas R. Clandinin
Genetics March 1, 2014 vol. 196 no. 3 615-623; https://doi.org/10.1534/genetics.113.159483
Daryl M. Gohl
Department of Neurobiology, Stanford University, Stanford, California 94305
Limor Freifeld
Department of Electrical Engineering, Stanford University, Stanford, California 94305Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Marion Silies
Department of Neurobiology, Stanford University, Stanford, California 94305
Jennifer J. Hwa
Department of Neurobiology, Stanford University, Stanford, California 94305
Mark Horowitz
Department of Electrical Engineering, Stanford University, Stanford, California 94305
Thomas R. Clandinin
Department of Neurobiology, Stanford University, Stanford, California 94305